Protocols
Methods used in the project are presented here. Some were developed specifically for the project and some have been optimised from published procedures.
- Calculation of cell number
- Chromatin immunoprecipitation
- Fast culture sampling
- Metabolic labelling (Pulse only or Pulse-chase)
- Northern blotting
- PASE (Poly A-status-examination) analysis
- Primer extension
- Probe sequences for FISH - see supp data 4 from Alexander, R.D., et al. RNA (2010)
- RiboSys reporter sequences - see supp data 2 from Alexander, R.D., et al. RNA (2010)
- RNA extraction and copy number estimation by RT-qPCR - see supp data 3 from Alexander, R.D., et al. RNA (2010)
- RNaseH analysis
- Splicing and 3′ end cleavage assay by RT-qPCR - see Alexander, R.D., et al. RNA (2010) doi:10.1261/rna.2162610
- Transcription run-on
- Visualisation of single transcripts
- Yeast RNA extraction
- Yeast strains, plasmids and oligonucleotides - see supp data 1 from Alexander, R.D., et al. RNA (2010)
Disclaimer: These protocols are made freely available in good faith, however, the authors and the EC cannot be liable for any use that may be made of them.